These nutrients are primarily in a nitrogen based nutrient form from fish waste. KoiZyme will consume these nutrients and thus starve Aeromonas and Psuedomonas. The bacteria counts of these pathogens will then be reduced to such a low level that they will not have an effect on our fish. KoiZyme should be used when pond water temps reach 52 degrees and above KoiZyme will NOT harm the biofilter and can be used with any percentage of salt Shelf life of KoiZyme: 4 to 6 months at room temps and 18 months if kept refrigerated